
                                SEQUENCE LISTING

<110> NETT, Juergen

<120> YEAST STRAINS FOR PROTEIN PRODUCTION
  

<130> GFI-BIO-0030

<150> 61/008,242          
<151> 2007-12-19  

<160> 62

<170> FastSEQ for Windows Version 4.0

<210> 1
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ura5comp5 primer

<400> 1
gctctagagg gacttatctg ggtccagacg atgtg                            35

<210> 2
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> Ura5comp3 primer

<400> 2
cgggatccgc cgccgtgccc aaagctccga aacag                            35

<210> 3
<211> 31
<212> DNA
<213> Artificial Sequence

<220> 
<223> URA5MUT1 primer

<400> 3
gcagtcatca catcatcgat aatcagtact c                                31

<210> 4
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> URA5MUT2 primer

<400> 4
ccgtgttgaa gttgtacgag ctgggcggc                                   29

<210> 5
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> URA5MUT3 primer

<400> 5
gccgcccagc tcgtacaact tcaacacgg                                   29

<210> 6
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> URA5MUT4 primer

<400> 6
cacattgaag atgtcactgg aggggtacc                                   29

<210> 7
<211> 29
<212> DNA
<213> Artificial Sequence

<220> 
<223> URA5MUT5 primer

<400> 7
ggtacccctc cagtgacatc ttcaatgtg                                   29

<210> 8
<211> 30
<212> DNA
<213> Artificial Sequence

<220> 
<223> URA5MUT6 primer

<400> 8
gctggctcgc gcgcagtgtt tttcgtgctc                                  30

<210> 9
<211> 46
<212> DNA
<213> Artificial Sequence

<220> 
<223> ARG355DIS primer

<400> 9
gagctcggcc agcttggccg ctaacagtaa caaaaactac cgccag                46

<210> 10
<211> 55
<212> DNA
<213> Artificial Sequence

<220> 
<223> ARG353-2 primer

<400> 10
gtcgacggat ccgtttaaac gacagccttc tttgggtcat gagtaacttc caaac      55

<210> 11
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> ARG335-2 primer

<400> 11
ggatccacta gtatttaaat cacggattta tgcttgatca catgaccaat cataac     56

<210> 12
<211> 43
<212> DNA
<213> Artificial Sequence

<220> 
<223> ARG333 primer

<400> 12
gtcgacggcc gatggggccc gcattcttct tgcttaataa acc                   43

<210> 13
<211> 48
<212> DNA
<213> Artificial Sequence

<220> 
<223> GAP5CLEAN primer

<400> 13
ggatccctcg agagatcttt tttgtagaaa tgtcttggtg tcctcgtc              48

<210> 14
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> GAP3CLEAN primer

<400> 14
gcatgcacta gtgcggccgc tgtgttttga tagttgttca attgattgaa atag       54

<210> 15
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> CYC5CLEAN primer

<400> 15
gctagcttaa ttaaacaggc cccttttcct ttgtcgatat catg                  44

<210> 16
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> CYC3CLEAN primer

<400> 16
gcatgcggat cccttaagag ccggcagctt gcaaattaaa gccttcgagc gtcc       54

<210> 17
<211> 64
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE155L primer

<400> 17
ccaccgagct cggccaactc ggcctttttc aagttgatgc tatcttttat ggatattaag 60
ccag                                                              64

<210> 18
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE153L primer

<400> 18
ccaccgttta aacctccatg ccacccatct aatgttgatc aacg                  44

<210> 19
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE1KO35 primer

<400> 19
atttaaatat gattagtacc ctcctcgcct ttttcagac                        39

<210> 20
<211> 54
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE133L primer

<400> 20
ccaccgcatg cggccatgtt ggcccctctt ttaagcaact ctcttggtcc ttgg       54

<210> 21
<211> 40
<212> DNA
<213> Artificial Sequence

<220> 
<223> RCD534 primer

<400> 21
gatatcggcc ggccatttac aattagtaat attaaggtgg                       40

<210> 22
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> RCD535 primer

<400> 22
cttaagcgga ccggtttaaa cctactaagc gacgaaaacg ggagc                 45

<210> 23
<211> 53
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE255KO primer

<400> 23
ggatgagctc ggccagttgg gcccttaaaa tcatctgcct caccccaccg acc        53

<210> 24
<211> 52
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE253KO primer

<400> 24
ggatggccgg ccgacttgct aacctggctc tgccatagtt gaaaatacgt cg         52

<210> 25
<211> 56
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE235 primer

<400> 25
ggacgattta aatatttagt attgtttttt aatagatgta tatataatag tacacg     56

<210> 26
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE233 primer

<400> 26
ggacggtcga cggccatact ggcctgagat ggatttgaaa tgctc                 45

<210> 27
<211> 76
<212> DNA
<213> Artificial Sequence

<220> 
<223> EXMCS1 primer

<400> 27
aattgagatc tgaattcggt accatttaaa tggatccgcg gccgcttaat taaggcgcgc 60
caggccataa tggcct                                                 76

<210> 28
<211> 76
<212> DNA
<213> Artificial Sequence

<220> 
<223> EXMCS2 primer

<400> 28
agctaggcca ttatggcctg gcgcgcctta attaagcggc cgcggatcca tttaaatggt 60
accgaattca gatctc                                                 76

<210> 29
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> CYCTT5 primer

<400> 29
gcaaggattt aaatacaggc cccttttcct ttgtcgatat catg                  44

<210> 30
<211> 35
<212> DNA
<213> Artificial Sequence

<220> 
<223> CYCTT3 primer

<400> 30
ggatccagct tgcaaattaa agccttcgag cgtcc                            35

<210> 31
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> AOX1P-5 primer

<400> 31
agatctaaca tccaaagacg aaaggttgaa tgaaacc                          37

<210> 32
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> AOX1P-3 primer

<400> 32
gaattccgtt tcgaataatt agttgttttt tgatcttc                         38

<210> 33
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRP2-5 primer

<400> 33
ggccataatg gccaaacggt ttctcaatta ctatatacta ctaac                 45

<210> 34
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> TRP2-3revised primer

<400> 34
ggccattatg gccaaaccat aaattcctac ttacgtcctc cg                    42

<210> 35
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE1-3 primer

<400> 35
ggcgcgccct gagccaaaag accccctgcc aatgagc                          37

<210> 36
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE1-5C-BAM primer

<400> 36
gcggccgcgg gtgctatcgt tttgtgcaat ttggtttgc                        39

<210> 37
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE1-5-100 primer

<400> 37
gcggccgcac ttttaccaat aatcgtttat gaatacgg                         38

<210> 38
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE1-5-186 primer

<400> 38
gcggccgctc cacttgaacg attcattatt caga                             34

<210> 39
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE1-5-295 primer

<400> 39
gcggccgccc aatatactac tctaggaaac tcgaaaaac                        39

<210> 40
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE1-5-325 primer

<400> 40
gcggccgccc tttccatgtg tcatcgcttc caacacac                         38

<210> 41
<211> 39
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE1-5-ORF primer

<400> 41
ttaattaaat gtccattgtg aacactgatc tggacggaa                        39

<210> 42
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> GAPDHP-5 primer

<400> 42
agatcttttt tgtagaaatg tcttggtgtc ctcgtcc                          37

<210> 43
<211> 34
<212> DNA
<213> Artificial Sequence

<220> 
<223> GAPDHP-3 primer

<400> 43
gaattctgtg ttttgatagt tgttcaattg attg                             34

<210> 44
<211> 42
<212> DNA
<213> Artificial Sequence

<220> 
<223> 5oligoERSSKFS primer

<400> 44
aattccggac cggcatgcag gcctggtacc ggccggccat tt                    42

<210> 45
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> 3oligoSFSSRF primer

<400> 45
aaatggccgg ccggtaccag gcctgcatgc cggtccgg                         38

<210> 46
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE23AscI primer

<400> 46
ggatggcgcg ccgcacatga ggctctttgc aaagttcctc cagg                  44

<210> 47
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE25NotI-1 primer

<400> 47
ggatgcggcc gcgtcaaagc cgtatactcg gtagtgtgct cgcc                  44

<210> 48
<211> 45
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE25NotI-2 primer

<400> 48
ggatgcggcc gcgacttgac tcttcactag cctatgcaaa taagg                 45

<210> 49
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE25NotI-3 primer

<400> 49
ggatgcggcc gcggttacct tttccaagaa tcgtagaaac gatt                  44

<210> 50
<211> 44
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE25NotI-4 primer

<400> 50
ggatgcggcc gccttccaaa ctctcatgga ttctcaggta atag                  44

<210> 51
<211> 59
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE2-5'PacInew primer

<400> 51
ggccttaatt aaatggattc tcaggtaata ggtattctag gaggaggcca gctaggccg  59

<210> 52
<211> 37
<212> DNA
<213> Artificial Sequence

<220> 
<223> AOX1P-5 primer

<400> 52
agatctaaca tccaaagacg aaaggttgaa tgaaacc                          37

<210> 53
<211> 38
<212> DNA
<213> Artificial Sequence

<220> 
<223> AOX1P-3 primer

<400> 53
gaattccgtt tcgaataatt agttgttttt tgatcttc                         38

<210> 54
<211> 1831
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE 1 5' seq for Knockout

<400> 54
tttttcaagt tgatgctatc ttttatggat attaagccag tgaaacttag agttagcagt 60
atcttatcaa gagtgaaaaa gttgtgtttc ttttcatttg aattgtgctt ggtcattgat 120
gaaatcagag tcattctcaa gatgtataac catatcgatc tataagtcgc agttgcttcc 180
aagtttgact cttgctcaat atccagatct atggaatctt gagcaggtct tttggaataa 240
aatgcgacta aaaacccaga aagtagccca attatatgca gtctgaacat gagtggtact 300
ttggtgagtg acctccatat ccatgacatg gatggatttc gcccttttct tgtgtaatat 360
gacatcaaca acgacgtgga tgacacagta acaacagtca aggagagttt gagactttct 420
tttacgcttt ttatgactat ctgtttgtaa tacttccatt tgctagccgc tttcagctgt 480
tccaattctt ccgtgctaag tctcaagttc ataaagaaga aaaatggaaa gaggtattca 540
aggactaccg tgtattttct tggcaaatat cgcaacagaa agtttctcag atcaaatgca 600
aatcgatttt tcatgctatt cttaccaatt atgctttcca gttcatagaa agatttgacc 660
atatcaccag atgaaaccat gcgagaagtt cctcttttga ctaataggcc ttcacccata 720
aagtttaaga tgttcctgaa atatactgga cagttctcgt aatccatgat aaacgacttg 780
aaaatctgcg agtaacataa tgggaataga taccatgaac gtaagagttt gtctctcttt 840
ggaacacttt ttagcgcttt gagcctacga atgaaacaac tattttctgg ttgatcttcg 900
aattcagcgt tgtctgtgtc tttcatatca gaatccttga taacgtatat agaggatgtc 960
tctttggaaa attggtcggg gtaaacctgt tccaagaact tatagccata ctctaccatt 1020
aataccgtaa aatatattga tgcataattc ttttggtaat atattttact gggatacagg 1080
gcaaatgaca ccactgatgt gaatagactg gaaacgactg aattgaaaag aaacttttgc 1140
ttctcagtga cttttaaata gctctctgcg aaaatgtcaa gaatcttgtt gaacaatggt 1200
ttaactgaaa ataagagacc cagtgatgta gaaaatttta gcaaattcac ccgatcattg 1260
aacattaaat ttcttctaga atttgcaata ttcaactttc ttaagatctt aaatattacg 1320
cccaacgatc caaacaacaa tagaaaccat ctgttgaagt ttctagctgc ctttatggtg 1380
acttttagta ttcctgttgt gtcgttctca taaaatgact gttctacagt cgataataag 1440
ccactcatct tccacaactt caactgcact tcctccaatg caactagatc atgcttttca 1500
agctgcttga gattgatctt cagtaattct ttaacttcat cgtgtgatgt gagcaagacg 1560
agtaaatact tgagttttgt caagttatta ctgcccttgt ttgacatgga ttgctgtatt 1620
tgagaagaaa aatgaacgta aacttgaatc tccccaggtg aacttggcgt gtatcttatc 1680
taccccagct ctaaagttta cccgatgagg taattcttag ggataatttg gtgtatggat 1740
ttgactaaat tgccggagtt gattcaatga cagagaagct tacatgcaag gaacatgatt 1800
cgttgatcaa cattagatgg gtggcatgga g                                1831

<210> 55
<211> 1444
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE 1 3' seq for  Knockout

<400> 55
atgattagta ccctcctcgc ctttttcaga catctgaaat ttcccttatt cttccaattc 60
catataaaat cctatttagg taattagtaa acaatgatca taaagtgaaa tcattcaagt 120
aaccattccg tttatcgttg atttaaaatc aataacgaat gaatgtcggt ctgagtagtc 180
aatttgttgc cttggagctc attggcaggg ggtcttttgg ctcagtatgg aaggttgaaa 240
ggaaaacaga tggaaagtgg ttcgtcagaa aagaggtatc ctacatgaag atgaatgcca 300
aagagatatc tcaagtgata gctgagttca gaattcttag tgagttaagc catcccaaca 360
ttgtgaagta ccttcatcac gaacatattt ctgagaataa aactgtcaat ttatacatgg 420
aatactgtga tggtggagat ctctccaagc tgattcgaac acatagaagg aacaaagagt 480
acatttcaga agaaaaaata tggagtattt ttacgcaggt tttattagca ttgtatcgtt 540
gtcattatgg aactgatttc acggcttcaa aggagtttga atcgctcaat aaaggtaata 600
gacgaaccca gaatccttcg tgggtagact cgacaagagt tattattcac agggatataa 660
aacccgacaa catctttctg atgaacaatt caaaccttgt caaactggga gattttggat 720
tagcaaaaat tctggaccaa gaaaacgatt ttgccaaaac atacgtcggt acgccgtatt 780
acatgtctcc tgaagtgctg ttggaccaac cctactcacc attatgtgat atatggtctc 840
ttgggtgcgt catgtatgag ctatgtgcat tgaggcctcc ttttcaagcc actacacatt 900
tacaattaca acaaaagatc caagaaggga cattccctcc acttccggac gtattttcac 960
cccggttaag atctctgatc aatgcttgca taaccataga cctgaaccaa cgaccatcta 1020
ctcacgaact tcttcaggaa agttgcttca atgtgtatat caaggaggtt aatttagaga 1080
taagggagga cagattgaat gagcgtgaac gcaaactgaa aatacgagag aacaagttaa 1140
tcttgagcga agagggaata gtgaaacaac tgaatgaaga actggaattt caaagaaagt 1200
tgcttgaaca agaagtagag gaaataagga agtcatacaa gaacgaattt cagttcgtac 1260
tggaacaaca ggtgcaacag gcattgagca aaattctagg tccccaatac aatcaaaagc 1320
cattgaacag gaatcagcaa caaaaacaaa tacaacaaat ttacagcaga caggatccgc 1380
aattatcaag cccaaagtca caacaagctc agatccaagg accaagagag ttgcttaaaa 1440
gagg                                                              1444

<210> 56
<211> 1510
<212> DNA
<213> Pichia pastoris

<400> 56
gggtgctatc gttttgtgca atttggtttg ctggagagtc gaccaagaga tgataactgt 60
tactaagctt ctccgtaatt agtggtattt tgtaactttt accaataatc gtttatgaat 120
acggatattt ttcgacctta tccagtgcca aatcacgtaa cttaatcatg gtttaaatac 180
tccacttgaa cgattcatta ttcagaaaaa agtcaggttg gcagaaacac ttgggcgctt 240
tgaagagtat aagagtatta agcattaaac atctgaactt tcaccgcccc aatatactac 300
tctaggaaac tcgaaaaatt cctttccatg tgtcatcgct tccaacacac tttgctgtat 360
ccttccaagt atgtccattg tgaacactga tctggacgga atcctacctt taatcgccaa 420
aggaaaggtt agagacattt atgcagtcga tgagaacaac ttgctgttcg tcgcaactga 480
ccgtatctcc gcttacgatg tgattatgac aaacggtatt cctgataagg gaaagatttt 540
gactcagctc tcagttttct ggtttgattt tttggcaccc tacataaaga atcatttggt 600
tgcttctaat gacaaggaag tctttgcttt actaccatca aaactgtctg aagaaaaata 660
caaatctcaa ttagagggac gatccttgat agtaaaaaag cacagactga tacctttgga 720
agccattgtc agaggttaca tcactggaag tgcatggaaa gagtacaaga actcaaaaac 780
tgtccatgga gtcaaggttg aaaacgagaa ccttcaagag agcgacgcct ttccaactcc 840
gattttcaca ccttcaacga aagctgaaca gggtgaacac gatgaaaaca tctctattga 900
acaagctgct gagattgtag gtaaagacat ttgtgagaag gtcgctgtca aggcggtcga 960
gttgtattct gctgcaaaaa acttcgccct tttgaagggg atcattattg ctgatacgaa 1020
attcgaattt ggactggacg aaaacaatga attggtacta gtagatgaag ttttaactcc 1080
agattcttct agattttgga atcaaaagac ttaccaagtg ggtaaatcgc aagagagtta 1140
cgataagcag tttctcagag attggttgac ggccaacgga ttgaatggca aagagggcgt 1200
agccatggat gcagaaattg ctatcaagag taaagaaaag tatattgaag cttatgaagc 1260
aattactggc aagaaatggg cttgaatgat tagtaccctc ctcgcctttt tcagacatct 1320
gaaatttccc ttattcttcc aattccatat aaaatcctat ttaggtaatt agtaaacaat 1380
gatcataaag tgaaatcatt caagtaacca ttccgtttat cgttgattta aaatcaataa 1440
cgaatgaatg tcggtctgag tagtcaattt gttgccttgg agctcattgg cagggggtct 1500
tttggctcag                                                        1510

<210> 57
<211> 304
<212> PRT
<213> Pichia pastoris

<400> 57
Met Ser Ile Val Asn Thr Asp Leu Asp Gly Ile Leu Pro Leu Ile Ala
 1               5                  10                  15      
Lys Gly Lys Val Arg Asp Ile Tyr Ala Val Asp Glu Asn Asn Leu Leu
            20                  25                  30          
Phe Val Ala Thr Asp Arg Ile Ser Ala Tyr Asp Val Ile Met Thr Asn
        35                  40                  45              
Gly Ile Pro Asp Lys Gly Lys Ile Leu Thr Gln Leu Ser Val Phe Trp
    50                  55                  60                  
Phe Asp Phe Leu Ala Pro Tyr Ile Lys Asn His Leu Val Ala Ser Asn
65                  70                  75                  80  
Asp Lys Glu Val Phe Ala Leu Leu Pro Ser Lys Leu Ser Glu Glu Lys
                85                  90                  95      
Tyr Lys Ser Gln Leu Glu Gly Arg Ser Leu Ile Val Lys Lys His Arg
            100                 105                 110         
Leu Ile Pro Leu Glu Ala Ile Val Arg Gly Tyr Ile Thr Gly Ser Ala
        115                 120                 125             
Trp Lys Glu Tyr Lys Asn Ser Lys Thr Val His Gly Val Lys Val Glu
    130                 135                 140                 
Asn Glu Asn Leu Gln Glu Ser Asp Ala Phe Pro Thr Pro Ile Phe Thr
145                 150                 155                 160 
Pro Ser Thr Lys Ala Glu Gln Gly Glu His Asp Glu Asn Ile Ser Ile
                165                 170                 175     
Glu Gln Ala Ala Glu Ile Val Gly Lys Asp Ile Cys Glu Lys Val Ala
            180                 185                 190         
Val Lys Ala Val Glu Leu Tyr Ser Ala Ala Lys Asn Phe Ala Leu Leu
        195                 200                 205             
Lys Gly Ile Ile Ile Ala Asp Thr Lys Phe Glu Phe Gly Leu Asp Glu
    210                 215                 220                 
Asn Asn Glu Leu Val Leu Val Asp Glu Val Leu Thr Pro Asp Ser Ser
225                 230                 235                 240 
Arg Phe Trp Asn Gln Lys Thr Tyr Gln Val Gly Lys Ser Gln Glu Ser
                245                 250                 255     
Tyr Asp Lys Gln Phe Leu Arg Asp Trp Leu Thr Ala Asn Gly Leu Asn
            260                 265                 270         
Gly Lys Glu Gly Val Ala Met Asp Ala Glu Ile Ala Ile Lys Ser Lys
        275                 280                 285             
Glu Lys Tyr Ile Glu Ala Tyr Glu Ala Ile Thr Gly Lys Lys Trp Ala
    290                 295                 300                 


<210> 58
<211> 1016
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE 2 5' seq for Knockout

<400> 58
agttgggccc ttaaaatcat ctgcctcacc ccaccgacca atgggaattc tagaaacaat 60
ttcattgctc ttcttctcgt taccataaga atcggctgtc atgtttgact taacgaaccc 120
tggaacaagg gaattcacgg taataccttt tggagcaagt tcaaccgata gagccttcat 180
taatgagttg attgcacctt tggtggtcgc atataccgat tgattcgggt aggtcacttc 240
gaaactgtac agggaggcag taaagatgat cctaccctta atctggttct taataaagtg 300
tttagtgact agctgtgtca atctaaatgg aaaatcgaca tttacctttt ggatagccgc 360
gtaatctttc tccgtaaaac ttgtaaactc agatttaatg gcaatggcag cgttgttgat 420
taaaatgtca atctttccag tggaactctt ctccaccgca ggactcgtta cggtctcttc 480
cagctttgca agatcggcat ccactagatc caactcaatt gtatgtatgg aggcaccatc 540
ggcatttgac attctcacct cttcaatgaa agccgttggg tctgtagaag gtctatggat 600
aagaataagt tctgcacctg cttcataaag tcctcgaact attccttggc ctaatccgct 660
ggtaccaccg gtgatcaagg cgaccttacc attcaaagaa aacaaatcag cggacattag 720
cgacttgaat agggaatggg ttagacaaat gaaagccgac gagccagcac tttatagtaa 780
gtgcaggtga gtcaataaga ataaatgtat ggcttgctgt ccctatcgcg taagaagctt 840
actaagatcg cctaaattga aaagttgaac aaatcagttc tagctggcct ccatcagcat 900
ttcgttctcc tctgatcatc tttgccaatc gctagcatgc cctcagcgtg caaggaaaag 960
cacgcttctt tcttatcgac gtattttcaa ctatggcaga gccaggttag caagtc     1016

<210> 59
<211> 1676
<212> DNA
<213> Artificial Sequence

<220> 
<223> ADE 2 3' seq for Knockout

<400> 59
atttagtatt gttttttaat agatgtatat ataatagtac acgtaactta tctattccat 60
tcataatttt attttaaagg ttcggtagaa atttgtcctc caaaaagttg gttagagcct 120
ggcagttttg ataggcatta ttatagattg ggtaatattt accctgcacc tggaggaact 180
ttgcaaagag cctcatgtgc tctaaaagga tgtcagaatt ccaacatttc aaaattatat 240
ctgcatgcgt ctgtaatact ggaactgtta tttttctggt caggatttca ccgctcttgt 300
cgtcatgttt ctcgtcgtct gaaagtaaac tgactttcct ctttccataa acacaaaaat 360
cgattgcaac ttggttattc ttgagattga aatttgctgt gtcttcagtg cttagctgaa 420
tatcaacaaa cttacttagt actaataacg aagcactatg gtaagtggca taacatagtg 480
gtattgaagc gaacagtgga tattgaaccc aagcattggc aacatctggc tctgttgata 540
ctgatccgga tcgtttggca ccaattcctg aaacggcgta gtgccaccaa ggtttcgatt 600
tgagaacagg ttcatcatca gagtcaacca ccccaatgtc aatggcaggc tccaacgaag 660
taggtccaac aacaacagga agtatttgac cttgaagatc tgttccttta tgatccacca 720
caccttgccc caattccaat aactttacca gtcccgatgc agacatgata actggtacta 780
atgatctcca ttgattttcg tcggcactac gtaaagcctc caaaaatgaa ttcagaatat 840
cttctgaaac tagattctgc ttctgtgatt caagcattgc tttatgtaga catctcttga 900
ataaaagcaa ttctccacat attggtgtgt gtaagataga tctggaaaga tgtatctgga 960
atagtccagt caacgttgtg caattgatta gcattacctt actgtgaaca tctctatcta 1020
caacaacaga ctcaattcga tagacgttcc gggaaagttt ttcaagcgca ttcagtttgc 1080
tgttgaacaa agtgactttg ctttccaatg tgcaaatacc cctgtatatc aagtccatca 1140
catcactcaa gaccttggtg gaaaagaatg aaacagctgg agcataattt tcgaatgaat 1200
taggtaaggt cacttcatcc ttatctgttg taatgctata atcaatagcg gaactaacat 1260
cttcccatgt aacaggtttc ttgatctctg aatctgaatc tttatttgaa aaagaattga 1320
aaaaagactc atcactcatt gggaattcaa ggtcattagg gtattccatt gttagttctg 1380
gtctaggttt aaagggatca ccttcgttaa gacgatggaa aatagctaat ctgtacaata 1440
accagatact tctaacgaag ctctctctat ccatcagttg acgtgttgag gatatctgaa 1500
ctagctcttt ccactgcgaa tcaggcatgc tcgtatagct ggcaagcatg ttattcagct 1560
ttaccaagtt agaagccctt tggaaaccat ctatagattc ccgaaaaaac ttatacccac 1620
tgagggtttc actgagcata gtcagtgaca tcaaagagca tttcaaatcc atctca     1676

<210> 60
<211> 2018
<212> DNA
<213> Pichia pastoris

<400> 60
gtcaaagccg tatactcggt agtgtgctcg ccaaaaataa atttgacttg actcttcact 60
agcctatgca aataaggtta ccttttccaa gaatcgtaga aacgattaaa aaacttccaa 120
actctcatgg attctcaggt aataggtatt ctaggaggag gccagctagg ccgaatgatt 180
gttgaggccg ctagcaggct caatatcaag accgtgattc ttgatgatgg tttttcacct 240
gctaagcaca ttaatgctgc gcaagaccac atcgacggat cattcaaaga tgaggaggct 300
atcgccaagt tagctgccaa atgtgatgtt ctcactgtag agattgagca tgtcaacaca 360
gatgctctaa agagagttca agacagaact ggaatcaaga tatatccttt accagagaca 420
atcgaactaa tcaaggataa gtacttgcaa aaggaacatt tgatcaagca caacatttcg 480
gtgacaaagt ctcagggtat agaatctaat gaaaaggcgc tgcttttgtt tggagaagag 540
aatggatttc catatctgtt gaagtcccgg actatggctt atgatggaag aggcaatttt 600
gtagtggagt ctaaagagga catcagtaag gcattagaat tcttgaaaga tcgtccattg 660
tatgccgaga agtttgctcc ttttgttaaa gaattagcgg taatggttgt gagatcactg 720
gaaggcgaag tattctccta cccaaccgta gaaactgtgc acaaggacaa tatctgtcat 780
attgtgtatg ctccggccag agttaatgac accatccaaa agaaagctca aatattagct 840
gaaaacactg tgaagacttt cccaggcgct ggaatcttcg gagttgagat gttcctattg 900
tctgatggag aacttcttgt aaatgagatt gctccaaggc cccacaattc tggtcactat 960
acaatcgatg catgtgtaac atctcagttc gaagcacatg taagagccat aactggtctg 1020
ccaatgccac tagatttcac caaactatct acttccaaca ccaacgctat tatgctcaat 1080
gttttgggtg ctgaaaaatc tcacggggaa ttagagtttt gtagaagagc cttagaaaca 1140
cccggtgctt ctgtatatct gtacggaaag accacccgat tggctcgtaa gatgggtcat 1200
atcaacataa taggatcttc catgttggaa gcagaacaaa agttagagta cattctagaa 1260
gaatcaaccc acttaccatc cagtactgta tcagctgaca ctaaaccgtt ggttggagtt 1320
atcatgggtt cagactctga tctacctgtg atttcgaaag gttgcgatat tttaaaacag 1380
tttggtgttc cattcgaagt tactattgtc tctgctcata gaacaccaca gagaatgacc 1440
agatatgcct ttgaagccgc tagtagaggt atcaaggcta tcattgcagg tgctggtggt 1500
gctgctcatc ttccaggaat ggttgctgcc atgactccgt tgccagtcat tggtgttcct 1560
gtcaagggct ctacgttgga tggtgtagac tcgctacact cgattgtcca aatgcctaga 1620
ggtgttcctg tggctacggt tgctatcaac aacgccacca atgccgctct gttggccatc 1680
aggattttag gtacaattga ccacaaatgg caaaaggaaa tgtccaagta tatgaatgca 1740
atggagaccg aagtgttggg gaaggcatcc aacttggaat ctgaagggta tgaatcctat 1800
ttgaagaatc gtctttgaat ttagtattgt tttttaatag atgtatatat aatagtacac 1860
gtaacttatc tattccattc ataattttat tttaaaggtt cggtagaaat ttgtcctcca 1920
aaaagttggt tagagcctgg cagttttgat aggcattatt atagattggg taatatttac 1980
cctgcacctg gaggaacttt gcaaagagcc tcatgtgc                         2018

<210> 61
<211> 563
<212> PRT
<213> Pichia pastoris

<400> 61
Met Asp Ser Gln Val Ile Gly Ile Leu Gly Gly Gly Gln Leu Gly Arg
 1               5                  10                  15      
Met Ile Val Glu Ala Ala Ser Arg Leu Asn Ile Lys Thr Val Ile Leu
            20                  25                  30          
Asp Asp Gly Phe Ser Pro Ala Lys His Ile Asn Ala Ala Gln Asp His
        35                  40                  45              
Ile Asp Gly Ser Phe Lys Asp Glu Glu Ala Ile Ala Lys Leu Ala Ala
    50                  55                  60                  
Lys Cys Asp Val Leu Thr Val Glu Ile Glu His Val Asn Thr Asp Ala
65                  70                  75                  80  
Leu Lys Arg Val Gln Asp Arg Thr Gly Ile Lys Ile Tyr Pro Leu Pro
                85                  90                  95      
Glu Thr Ile Glu Leu Ile Lys Asp Lys Tyr Leu Gln Lys Glu His Leu
            100                 105                 110         
Ile Lys His Asn Ile Ser Val Thr Lys Ser Gln Gly Ile Glu Ser Asn
        115                 120                 125             
Glu Lys Ala Leu Leu Leu Phe Gly Glu Glu Asn Gly Phe Pro Tyr Leu
    130                 135                 140                 
Leu Lys Ser Arg Thr Met Ala Tyr Asp Gly Arg Gly Asn Phe Val Val
145                 150                 155                 160 
Glu Ser Lys Glu Asp Ile Ser Lys Ala Leu Glu Phe Leu Lys Asp Arg
                165                 170                 175     
Pro Leu Tyr Ala Glu Lys Phe Ala Pro Phe Val Lys Glu Leu Ala Val
            180                 185                 190         
Met Val Val Arg Ser Leu Glu Gly Glu Val Phe Ser Tyr Pro Thr Val
        195                 200                 205             
Glu Thr Val His Lys Asp Asn Ile Cys His Ile Val Tyr Ala Pro Ala
    210                 215                 220                 
Arg Val Asn Asp Thr Ile Gln Lys Lys Ala Gln Ile Leu Ala Glu Asn
225                 230                 235                 240 
Thr Val Lys Thr Phe Pro Gly Ala Gly Ile Phe Gly Val Glu Met Phe
                245                 250                 255     
Leu Leu Ser Asp Gly Glu Leu Leu Val Asn Glu Ile Ala Pro Arg Pro
            260                 265                 270         
His Asn Ser Gly His Tyr Thr Ile Asp Ala Cys Val Thr Ser Gln Phe
        275                 280                 285             
Glu Ala His Val Arg Ala Ile Thr Gly Leu Pro Met Pro Leu Asp Phe
    290                 295                 300                 
Thr Lys Leu Ser Thr Ser Asn Thr Asn Ala Ile Met Leu Asn Val Leu
305                 310                 315                 320 
Gly Ala Glu Lys Ser His Gly Glu Leu Glu Phe Cys Arg Arg Ala Leu
                325                 330                 335     
Glu Thr Pro Gly Ala Ser Val Tyr Leu Tyr Gly Lys Thr Thr Arg Leu
            340                 345                 350         
Ala Arg Lys Met Gly His Ile Asn Ile Ile Gly Ser Ser Met Leu Glu
        355                 360                 365             
Ala Glu Gln Lys Leu Glu Tyr Ile Leu Glu Glu Ser Thr His Leu Pro
    370                 375                 380                 
Ser Ser Thr Val Ser Ala Asp Thr Lys Pro Leu Val Gly Val Ile Met
385                 390                 395                 400 
Gly Ser Asp Ser Asp Leu Pro Val Ile Ser Lys Gly Cys Asp Ile Leu
                405                 410                 415     
Lys Gln Phe Gly Val Pro Phe Glu Val Thr Ile Val Ser Ala His Arg
            420                 425                 430         
Thr Pro Gln Arg Met Thr Arg Tyr Ala Phe Glu Ala Ala Ser Arg Gly
        435                 440                 445             
Ile Lys Ala Ile Ile Ala Gly Ala Gly Gly Ala Ala His Leu Pro Gly
    450                 455                 460                 
Met Val Ala Ala Met Thr Pro Leu Pro Val Ile Gly Val Pro Val Lys
465                 470                 475                 480 
Gly Ser Thr Leu Asp Gly Val Asp Ser Leu His Ser Ile Val Gln Met
                485                 490                 495     
Pro Arg Gly Val Pro Val Ala Thr Val Ala Ile Asn Asn Ala Thr Asn
            500                 505                 510         
Ala Ala Leu Leu Ala Ile Arg Ile Leu Gly Thr Ile Asp His Lys Trp
        515                 520                 525             
Gln Lys Glu Met Ser Lys Tyr Met Asn Ala Met Glu Thr Glu Val Leu
    530                 535                 540                 
Gly Lys Ala Ser Asn Leu Glu Ser Glu Gly Tyr Glu Ser Tyr Leu Lys
545                 550                 555                 560 
Asn Arg Leu
            


<210> 62
<211> 571
<212> PRT
<213> Saccharomyces cereviseae

<400> 62
Met Asp Ser Arg Thr Val Gly Ile Leu Gly Gly Gly Gln Leu Gly Arg
 1               5                  10                  15      
Met Ile Val Glu Ala Ala Asn Arg Leu Asn Ile Lys Thr Val Ile Leu
            20                  25                  30          
Asp Ala Glu Asn Ser Pro Ala Lys Gln Ile Ser Asn Ser Asn Asp His
        35                  40                  45              
Val Asn Gly Ser Phe Ser Asn Pro Leu Asp Ile Glu Lys Leu Ala Glu
    50                  55                  60                  
Lys Cys Asp Val Leu Thr Ile Glu Ile Glu His Val Asp Val Pro Thr
65                  70                  75                  80  
Leu Lys Asn Leu Gln Val Lys His Pro Lys Leu Lys Ile Tyr Pro Ser
                85                  90                  95      
Pro Glu Thr Ile Arg Leu Ile Gln Asp Lys Tyr Ile Gln Lys Glu His
            100                 105                 110         
Leu Ile Lys Asn Gly Ile Ala Val Thr Gln Ser Val Pro Val Glu Gln
        115                 120                 125             
Ala Ser Glu Thr Ser Leu Leu Asn Val Gly Arg Asp Leu Gly Phe Pro
    130                 135                 140                 
Phe Val Leu Lys Ser Arg Thr Leu Ala Tyr Asp Gly Arg Gly Asn Phe
145                 150                 155                 160 
Val Val Lys Asn Lys Glu Met Ile Pro Glu Ala Leu Glu Val Leu Lys
                165                 170                 175     
Asp Arg Pro Leu Tyr Ala Glu Lys Trp Ala Pro Phe Thr Lys Glu Leu
            180                 185                 190         
Ala Val Met Ile Val Arg Ser Val Asn Gly Leu Val Phe Ser Tyr Pro
        195                 200                 205             
Ile Val Glu Thr Ile His Lys Asp Asn Ile Cys Asp Leu Cys Tyr Ala
    210                 215                 220                 
Pro Ala Arg Val Pro Asp Ser Val Gln Leu Lys Ala Lys Leu Leu Ala
225                 230                 235                 240 
Glu Asn Ala Ile Lys Ser Phe Pro Gly Cys Gly Ile Phe Gly Val Glu
                245                 250                 255     
Met Phe Tyr Leu Glu Thr Gly Glu Leu Leu Ile Asn Glu Ile Ala Pro
            260                 265                 270         
Arg Pro His Asn Ser Gly His Tyr Thr Ile Asp Ala Cys Val Thr Ser
        275                 280                 285             
Gln Phe Glu Ala His Leu Arg Ser Ile Leu Asp Leu Pro Met Pro Lys
    290                 295                 300                 
Asn Phe Thr Ser Phe Ser Thr Ile Thr Thr Asn Ala Ile Met Leu Asn
305                 310                 315                 320 
Val Leu Gly Asp Lys His Thr Lys Asp Lys Glu Leu Glu Thr Cys Glu
                325                 330                 335     
Arg Ala Leu Ala Thr Pro Gly Ser Ser Val Tyr Leu Tyr Gly Lys Glu
            340                 345                 350         
Ser Arg Pro Asn Arg Lys Val Gly His Ile Asn Ile Ile Ala Ser Ser
        355                 360                 365             
Met Ala Glu Cys Glu Gln Arg Leu Asn Tyr Ile Thr Gly Arg Thr Asp
    370                 375                 380                 
Ile Pro Ile Lys Ile Ser Val Ala Gln Lys Leu Asp Leu Glu Ala Met
385                 390                 395                 400 
Val Lys Pro Leu Val Gly Ile Ile Met Gly Ser Asp Ser Asp Leu Pro
                405                 410                 415     
Val Met Ser Ala Ala Cys Ala Val Leu Lys Asp Phe Gly Val Pro Phe
            420                 425                 430         
Glu Val Thr Ile Val Ser Ala His Arg Thr Pro His Arg Met Ser Ala
        435                 440                 445             
Tyr Ala Ile Ser Ala Ser Lys Arg Gly Ile Lys Thr Ile Ile Ala Gly
    450                 455                 460                 
Ala Gly Gly Ala Ala His Leu Pro Gly Met Val Ala Ala Met Thr Pro
465                 470                 475                 480 
Leu Pro Val Ile Gly Val Pro Val Lys Gly Ser Cys Leu Asp Gly Val
                485                 490                 495     
Asp Ser Leu His Ser Ile Val Gln Met Pro Arg Gly Val Pro Val Ala
            500                 505                 510         
Thr Val Ala Ile Asn Asn Ser Thr Asn Ala Ala Leu Leu Ala Val Arg
        515                 520                 525             
Leu Leu Gly Ala Tyr Asp Ser Ser Tyr Thr Thr Lys Met Glu Gln Phe
    530                 535                 540                 
Leu Leu Lys Gln Glu Glu Glu Val Leu Val Lys Ala Gln Lys Leu Glu
545                 550                 555                 560 
Thr Val Gly Tyr Glu Ala Tyr Leu Glu Asn Lys
                565                 570     

